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falcon flow cytometry injection tube  (Becton Dickinson)

 
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    Becton Dickinson falcon flow cytometry injection tube
    Falcon Flow Cytometry Injection Tube, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/flow+cytometry+injection+tube/falcon+flow+cytometry+injection+tube/us10443041-166-208-206
    Average 90 stars, based on 1 article reviews
    falcon flow cytometry injection tube - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Marker:

    Article Title: NKT-like cell subpopulation and method of using the same in the treatment of tumor
    Article Snippet: The experimental steps were specifically listed as follows: i) the spleen mononuclear cells were resuspended in PBS buffer solution to 108/mL; ii) CD49b-PE (CD49b is a marker of panNK positive cells) was added in an amount of 50 μL/mL cell suspension, and incubated away from light at room temperature for 15 minutes; iii) a cocktail liquid was added in an amount of 100 μL/mL cell suspension, and incubated away from light at room temperature for 15 minutes; iv) magnetic beads were added in an amount of 50 μL/mL cell suspension, 20 μL/mL TCRβ-FITC and CD8-APC-Cy7 were further added, and the resulting cell suspension was incubated away from light at room temperature for 10 minutes; v) PBS was added to the cell suspension to reach a total volume of 2.5 mL, and then the resulting cell suspension was transferred to a 5 mL BD Falcon flow cytometry injection tube which was subsequently placed in a Stemcell positive sorting magnet and let stand for 5 minutes; vi) the magnet was picked up and the negative cells were poured into a new centrifuge tube; vii) the cell pellet was resuspended in 2.5 mL PBS, and the resulting cell suspension was placed into a Stemcell positive sorting magnet and let stand for 5 minutes; viii) the magnet was picked up and the negative cells were discarded; and ix) the BD Falcon flow cytometry injection tube was taken from the magnet. (3) The positive cells were resuspended in 200 μL PBS, labeled with 10 μL APC labeled α-GalCer-loaded CD1d tetramer (Proimmune Inc.), and then incubated at 4° C. for 30 minutes. (4) The cells were washed one time with 1 mL PBS.

    Suspension:

    Article Title: NKT-like cell subpopulation and method of using the same in the treatment of tumor
    Article Snippet: The experimental steps were specifically listed as follows: i) the spleen mononuclear cells were resuspended in PBS buffer solution to 108/mL; ii) CD49b-PE (CD49b is a marker of panNK positive cells) was added in an amount of 50 μL/mL cell suspension, and incubated away from light at room temperature for 15 minutes; iii) a cocktail liquid was added in an amount of 100 μL/mL cell suspension, and incubated away from light at room temperature for 15 minutes; iv) magnetic beads were added in an amount of 50 μL/mL cell suspension, 20 μL/mL TCRβ-FITC and CD8-APC-Cy7 were further added, and the resulting cell suspension was incubated away from light at room temperature for 10 minutes; v) PBS was added to the cell suspension to reach a total volume of 2.5 mL, and then the resulting cell suspension was transferred to a 5 mL BD Falcon flow cytometry injection tube which was subsequently placed in a Stemcell positive sorting magnet and let stand for 5 minutes; vi) the magnet was picked up and the negative cells were poured into a new centrifuge tube; vii) the cell pellet was resuspended in 2.5 mL PBS, and the resulting cell suspension was placed into a Stemcell positive sorting magnet and let stand for 5 minutes; viii) the magnet was picked up and the negative cells were discarded; and ix) the BD Falcon flow cytometry injection tube was taken from the magnet. (3) The positive cells were resuspended in 200 μL PBS, labeled with 10 μL APC labeled α-GalCer-loaded CD1d tetramer (Proimmune Inc.), and then incubated at 4° C. for 30 minutes. (4) The cells were washed one time with 1 mL PBS.

    Incubation:

    Article Title: NKT-like cell subpopulation and method of using the same in the treatment of tumor
    Article Snippet: The experimental steps were specifically listed as follows: i) the spleen mononuclear cells were resuspended in PBS buffer solution to 108/mL; ii) CD49b-PE (CD49b is a marker of panNK positive cells) was added in an amount of 50 μL/mL cell suspension, and incubated away from light at room temperature for 15 minutes; iii) a cocktail liquid was added in an amount of 100 μL/mL cell suspension, and incubated away from light at room temperature for 15 minutes; iv) magnetic beads were added in an amount of 50 μL/mL cell suspension, 20 μL/mL TCRβ-FITC and CD8-APC-Cy7 were further added, and the resulting cell suspension was incubated away from light at room temperature for 10 minutes; v) PBS was added to the cell suspension to reach a total volume of 2.5 mL, and then the resulting cell suspension was transferred to a 5 mL BD Falcon flow cytometry injection tube which was subsequently placed in a Stemcell positive sorting magnet and let stand for 5 minutes; vi) the magnet was picked up and the negative cells were poured into a new centrifuge tube; vii) the cell pellet was resuspended in 2.5 mL PBS, and the resulting cell suspension was placed into a Stemcell positive sorting magnet and let stand for 5 minutes; viii) the magnet was picked up and the negative cells were discarded; and ix) the BD Falcon flow cytometry injection tube was taken from the magnet. (3) The positive cells were resuspended in 200 μL PBS, labeled with 10 μL APC labeled α-GalCer-loaded CD1d tetramer (Proimmune Inc.), and then incubated at 4° C. for 30 minutes. (4) The cells were washed one time with 1 mL PBS.

    Magnetic Beads:

    Article Title: NKT-like cell subpopulation and method of using the same in the treatment of tumor
    Article Snippet: The experimental steps were specifically listed as follows: i) the spleen mononuclear cells were resuspended in PBS buffer solution to 108/mL; ii) CD49b-PE (CD49b is a marker of panNK positive cells) was added in an amount of 50 μL/mL cell suspension, and incubated away from light at room temperature for 15 minutes; iii) a cocktail liquid was added in an amount of 100 μL/mL cell suspension, and incubated away from light at room temperature for 15 minutes; iv) magnetic beads were added in an amount of 50 μL/mL cell suspension, 20 μL/mL TCRβ-FITC and CD8-APC-Cy7 were further added, and the resulting cell suspension was incubated away from light at room temperature for 10 minutes; v) PBS was added to the cell suspension to reach a total volume of 2.5 mL, and then the resulting cell suspension was transferred to a 5 mL BD Falcon flow cytometry injection tube which was subsequently placed in a Stemcell positive sorting magnet and let stand for 5 minutes; vi) the magnet was picked up and the negative cells were poured into a new centrifuge tube; vii) the cell pellet was resuspended in 2.5 mL PBS, and the resulting cell suspension was placed into a Stemcell positive sorting magnet and let stand for 5 minutes; viii) the magnet was picked up and the negative cells were discarded; and ix) the BD Falcon flow cytometry injection tube was taken from the magnet. (3) The positive cells were resuspended in 200 μL PBS, labeled with 10 μL APC labeled α-GalCer-loaded CD1d tetramer (Proimmune Inc.), and then incubated at 4° C. for 30 minutes. (4) The cells were washed one time with 1 mL PBS.

    Flow Cytometry:

    Article Title: NKT-like cell subpopulation and method of using the same in the treatment of tumor
    Article Snippet: The experimental steps were specifically listed as follows: i) the spleen mononuclear cells were resuspended in PBS buffer solution to 108/mL; ii) CD49b-PE (CD49b is a marker of panNK positive cells) was added in an amount of 50 μL/mL cell suspension, and incubated away from light at room temperature for 15 minutes; iii) a cocktail liquid was added in an amount of 100 μL/mL cell suspension, and incubated away from light at room temperature for 15 minutes; iv) magnetic beads were added in an amount of 50 μL/mL cell suspension, 20 μL/mL TCRβ-FITC and CD8-APC-Cy7 were further added, and the resulting cell suspension was incubated away from light at room temperature for 10 minutes; v) PBS was added to the cell suspension to reach a total volume of 2.5 mL, and then the resulting cell suspension was transferred to a 5 mL BD Falcon flow cytometry injection tube which was subsequently placed in a Stemcell positive sorting magnet and let stand for 5 minutes; vi) the magnet was picked up and the negative cells were poured into a new centrifuge tube; vii) the cell pellet was resuspended in 2.5 mL PBS, and the resulting cell suspension was placed into a Stemcell positive sorting magnet and let stand for 5 minutes; viii) the magnet was picked up and the negative cells were discarded; and ix) the BD Falcon flow cytometry injection tube was taken from the magnet. (3) The positive cells were resuspended in 200 μL PBS, labeled with 10 μL APC labeled α-GalCer-loaded CD1d tetramer (Proimmune Inc.), and then incubated at 4° C. for 30 minutes. (4) The cells were washed one time with 1 mL PBS.

    Injection:

    Article Title: NKT-like cell subpopulation and method of using the same in the treatment of tumor
    Article Snippet: The experimental steps were specifically listed as follows: i) the spleen mononuclear cells were resuspended in PBS buffer solution to 108/mL; ii) CD49b-PE (CD49b is a marker of panNK positive cells) was added in an amount of 50 μL/mL cell suspension, and incubated away from light at room temperature for 15 minutes; iii) a cocktail liquid was added in an amount of 100 μL/mL cell suspension, and incubated away from light at room temperature for 15 minutes; iv) magnetic beads were added in an amount of 50 μL/mL cell suspension, 20 μL/mL TCRβ-FITC and CD8-APC-Cy7 were further added, and the resulting cell suspension was incubated away from light at room temperature for 10 minutes; v) PBS was added to the cell suspension to reach a total volume of 2.5 mL, and then the resulting cell suspension was transferred to a 5 mL BD Falcon flow cytometry injection tube which was subsequently placed in a Stemcell positive sorting magnet and let stand for 5 minutes; vi) the magnet was picked up and the negative cells were poured into a new centrifuge tube; vii) the cell pellet was resuspended in 2.5 mL PBS, and the resulting cell suspension was placed into a Stemcell positive sorting magnet and let stand for 5 minutes; viii) the magnet was picked up and the negative cells were discarded; and ix) the BD Falcon flow cytometry injection tube was taken from the magnet. (3) The positive cells were resuspended in 200 μL PBS, labeled with 10 μL APC labeled α-GalCer-loaded CD1d tetramer (Proimmune Inc.), and then incubated at 4° C. for 30 minutes. (4) The cells were washed one time with 1 mL PBS.

    Labeling:

    Article Title: NKT-like cell subpopulation and method of using the same in the treatment of tumor
    Article Snippet: The experimental steps were specifically listed as follows: i) the spleen mononuclear cells were resuspended in PBS buffer solution to 108/mL; ii) CD49b-PE (CD49b is a marker of panNK positive cells) was added in an amount of 50 μL/mL cell suspension, and incubated away from light at room temperature for 15 minutes; iii) a cocktail liquid was added in an amount of 100 μL/mL cell suspension, and incubated away from light at room temperature for 15 minutes; iv) magnetic beads were added in an amount of 50 μL/mL cell suspension, 20 μL/mL TCRβ-FITC and CD8-APC-Cy7 were further added, and the resulting cell suspension was incubated away from light at room temperature for 10 minutes; v) PBS was added to the cell suspension to reach a total volume of 2.5 mL, and then the resulting cell suspension was transferred to a 5 mL BD Falcon flow cytometry injection tube which was subsequently placed in a Stemcell positive sorting magnet and let stand for 5 minutes; vi) the magnet was picked up and the negative cells were poured into a new centrifuge tube; vii) the cell pellet was resuspended in 2.5 mL PBS, and the resulting cell suspension was placed into a Stemcell positive sorting magnet and let stand for 5 minutes; viii) the magnet was picked up and the negative cells were discarded; and ix) the BD Falcon flow cytometry injection tube was taken from the magnet. (3) The positive cells were resuspended in 200 μL PBS, labeled with 10 μL APC labeled α-GalCer-loaded CD1d tetramer (Proimmune Inc.), and then incubated at 4° C. for 30 minutes. (4) The cells were washed one time with 1 mL PBS.



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